The separation methods of papain such as affinity chromatography, ultrafiltration, deposition, solvent extraction, aqueous two-phase extraction and so on are evaluated. 对亲和层析法、超滤膜分离法、沉淀分离法、溶剂萃取法、双水相萃取法等分离提取木瓜蛋白酶的方法进行了评述;
Novel separation and purification technique& affinity ultrafiltration and its application 新型分离纯化技术&亲和超滤及其应用
It was found that treating Saccharomyces cerevisiae cells by chemical permeabilization and purification by affinity ultrafiltration gives NADH with high yields. 试验表明使用化学渗透法处理酵母细胞,用亲和超滤纯化可获得高的NADH产率。
Affinity ultrafiltration is a novel technology of the high selectivity of affinity chromatography combined with the high production capacity of ultrafiltration. It is used on a large scale in the separation and purification of biological characteristic substances. 亲和超滤技术是把亲和层析的高选择性和超滤技术的高处理能力相结合的一种新型能大规模进行生物特征物质分离提纯的技术。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography. 用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法,从牛胎盘中分离纯化出一种肝细胞生长因子。
Meanwhile, the fusion protein SEO-his was purified by affinity chromatography, anion exchange chromatography and ultrafiltration and identified by western blotting analysis. 通过亲和层析,阴离子层析,超滤对重组融合蛋白进行纯化,并通过蛋白质免疫印迹鉴定重组融合蛋白。
After renaturation of inclusion bodies, fusion proteins were purified by glutathione sepharose 4B affinity chromatography and cleaved by factor Xa. The recombinant peptides were further purified and concentrated by ultrafiltration. 包涵体复性后,首先经Glutathionesepharose4B亲和层析纯化,然后利用因子Xa切去担体蛋白GST,最后通过超滤进一步纯化和浓缩产物。